OD Scanner for Shake Flasks

OD Scanner for Shake Flasks

BugLab’s OD Scanner eliminates the need to open shake flasks to measure biomass. Simply point the sensor into a shake flask, and within 6 seconds, your biomass is complete. No more dilutions or wasted samples!

  • The world’s first and only handheld biomass monitor.
  • Measures biomass from shake flasks and culture bottles in seconds.
  • Completely noninvasive – measures through vessel wall.
  • No costly consumables.  No risk of contamination.
  • Measures 0-30 OD units without needing a dilution.
  • Built-in predefined biomass calibrations for E. coli, yeast, etc.

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The hand held OD Scanner has been demonstrated across a wide range of vessel sizes (250 to 2000 mL) and materials (glass, PC, PET).  The vessel wall must be less than 5 mm or less in wall thickness.

A minimum of 3 cm of fluid needs to be present in front of the scanner during measurement.  A 20% fill volume in a 250ml (or larger) flask, is typically suitable with a proper tilt of the flask.  (see video for demonstration)

Most mono-dispersed cell types can be measured.  The relationship between OD and biomass is non-linear for organisms that are not mono-disperse in a liquid culture (we should note that this is true for most conventional measuring means as well).  As a result, OD can only be expected to provide an accurate measure of biomass for mono-disperse liquid cultures.  For organisms that grow in clusters, OD can only be expected to provide a qualitative estimate of biomass.

The OD scanner has a measuring range from 0-10 g/l.  This has a correlation of approximately 0-30 OD units for most cell types (e.g. Saccharomyces Cerevisiae, E.Coli).

One of the advantages of using an infrared laser source for measuring biomass is the avoidance of light absorbance by colored media components (and colored vessel materials).  This is  useful for measuring biomass containing strongly visible-light-absorbing chromophores (e.g. photosynthetic algae).

The sensor will store up to 999 measurements.  At any given time, the user can download the data, via the USB, to our data logging software.  Please see take a look at the software from the products page.